Home Archive Vol.41, No.3, 2015 Endomicroscopy with Fluorescent CD105 Antibodies for ”In Vivo” Imaging of Colorectal Cancer Angiogenesis

Endomicroscopy with Fluorescent CD105 Antibodies for ”In Vivo” Imaging of Colorectal Cancer Angiogenesis

Adriana Ciocâlteu(1), D. PIRICI(2), Claudia Valentina Georgescu(3), V. Surlin(4), A. Săftoiu(5)

(1)Research Center of Gastroenterology and Hepatology, University of Medicine and Pharmacy of Craiova, Romania, (2)Department of Research Methodology, University of Medicine and Pharmacy of Craiova, Romania, (3)Department of Pathology, Emergency County Hospital, Craiova, Romania, (4)Department of Surgery, University of Medicine and Pharmacy of Craiova, Romania, (5)Research Center of Gastroenterology and Hepatology, University of Medicine and Pharmacy of Craiova, Romania; Endoscopy Department, Copenhagen University Hospital Herlev, Copenhagen, Denmark

    Abstract: The aim of this case report was to evaluate the feasibility of in vivo acquisition of microscopic images using fluorescent CD105 antibodies for molecular imaging in human colorectal cancer. After excluding the presence of tissue autofluorescence, the antibody solution was topically administered through a spray-catheter. The targeted area was analyzed by eCLE and images were recorded. The fractal dimension of tumor vessels and the vessel density were determined using ImageJ software. Immunohistochemistry was used as a gold standard. In vivo CLE analysis of CD105 expression enabled the study of tumor vascular network, revealing a chaotic structure.
    Keywords: CD105-FITC, colorectal cancer, confocal laser endomicroscopy (CLE), in vivo endoscopic imaging, neoangiogenesis

DOI 10.12865/CHSJ.41.03.17


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Volume 41 Issue 3 2015