Home Archive Vol.43, No.1, 2017 In vitro Research of the Concentration Dependence of Effect of Adrenaline on Platelets Aggregation

In vitro Research of the Concentration Dependence of Effect of Adrenaline on Platelets Aggregation

E. Mati(1), Alice Piperea-Sianu(1), I. SARBU(1), A. CROITORU(1), STILIYANA BORISOVA(1)

(1)“Carol Davila” University of Medicine and Pharmacy, Faculty of Pharmacy, Doctoral School, Bucharest, Romania

    Abstract: Purpose. Paper intended to present experimental evidences that adrenaline has a direct effect of inducing platelets aggregation in the concentration range 1-8µM. Material/ Methods. Platelet rich plasma from patients of Colentina Clinical Hospital, following an informed consent. The platelet rich plasma (PRP) was prepared by centrifuging the anticoagulated sample at 200 G for 10 minutes. Aggregation was evaluated by optical aggregometry, classical method of Born, using Helena PACKS-4 Aggregometer. Results. The curves transmission light-time followed the structure: a lag-time, a first phase aggregation, more or less linear, defined by a “Slope 1”, a second wave of aggregation defined by “slope 2” and a “saturation” phase. Slope 1 increases with the concentration of adrenaline. The second slopes of the aggregation curves, maximum aggregation and areas under curves depended linear on adrenaline concentration. Conclusions. Adrenaline, in concentrations in the 1-8µM, induce aggregation of human platelets from platelet rich plasma. Linear regression models for slope and area were practically identical suggesting a rather unique than biphasic mechanism of action of adrenaline during the time course of aggregation.
    Keywords: Adrenaline, Platelet Aggregation, DLVO

DOI 10.12865/CHSJ.43.01.06 


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Volume 43 Issue 1 2017