Home Archive Vol.43, No.4, 2017 Isolation of Collagen from Marine Resources from the Black Sea

Isolation of Collagen from Marine Resources from the Black Sea


(1)PhD-Student Pharmacyst, Doctoral School ”Carol Davila” University of Medicine and Pharmacy, Faculty of Pharmacy, Bucharest, Romania, (2)Faculty of Pharmacy, ”Ovidius” University of Constanta, Romania, (3)Faculty of Pharmacy, University of Medicine and Pharmacy of Craiova, Romania

    Abstract: Purpose: Evaluation of possibilities of obtaining type I fibrillar collagen from fish skin using the Black Sea marine resources and suitable cross-linking agent for this one to stay stable at body or room temperature. Materials and methods: Collagen has been extracted from the skin of the Grey mullet and Sturgeon by the acidic method with Acetic acid 0.5M. Cross-linking has been performed by using Tanic acid at 4-6oC. The rheological behaviour was determined by using a Haake VT 550 rheo-viscometer. Results: The collagen was isolated from the skin of Gray mullet and Sturgeon. (Acipenser gueldenstaedtii). Hydrogels have a pseudoplastic behavior. Tanic acid was used for cross-linking like a better alternative, eliminating the toxicity of glutaraldehyde. Conclusion. The yield of collagen extraction was higher for the Gray mullet skin, than Sturgeon. Pseudo-plastic behavior allows them to be successfully applied in the treatment of wounds. The isolated type I collagen may serve as an attractive alternative to mammalian collagen for biomedical and pharmaceutical applications.
    Keywords: marine collagen, collagen hydrolysates, tissue regeneration

DOI 10.12865/CHSJ.43.04.02 

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Volume 43 Issue 4 2017