CHSJ

Curr Health Sci J, vol. 41, no. 2, 2015

Evaluation of Angiogenesis in Colorectal Cancer

[Original Paper]

IONELA CRISTINA DELIU(1), PAULINA CIUREA(2), DANIELA NEAGOE(1), MARIA CRISTINA BEZNA(3), IOANA GHEONEA(4), C.D. USCATU(5), T. DUMITRESCU(6), T. CIUREA(1)


(1)Department of Gastroenterology, University of Medicine and Pharmacy of Craiova, Romania,
(2)Department of Reumathology, University of Medicine and Pharmacy of Craiova, Romania,
(3)Department of Cardiology, University of Medicine and Pharmacy of Craiova, Romania,
(4)Department of Radiology and Imaging, University of Medicine and Pharmacy of Craiova, Romania,
(5)Department of Cellular and Molecular Biology, University of Medicine and Pharmacy of Craiova, Romania,
(6)Department of Surgery, University of Medicine and Pharmacy of Craiova, Romania


Abstract:

Purpose: Angiogenesis is an important step in the process of cancer growth. The purpose of this study was to determine the neoangiogenesis with CD31, CD34 and CD105, and tried to observe the differences between these three antibodies. Material/Methods: The blood vessels stained with CD31, CD 34 and CD105 were counted, and we reported their number per square millimeter to obtain microvascular density (MVD). For angiogenesis quantification we determined the neoformation blood vessels with CD105. The CD31 and CD34 were used as control markers, in order to observe the difference between neoformation blood vessels and mature vessels. Results: Comparing the average effective vessels marked with the 3 markers, Student t test showed that the mean number of blood vessels market with CD 34 is higher than blood vessels market with CD31 and CD 105. The value of the Student t test was highly significant in all three cases (p<0.001). By calculating the Pearson correlation coefficient for the relationship CD31-CD105 we obtained a value r = 0.440, which corresponds to p = 0.0013 <0.05, indicating a statistically significant direct correlation between the two factors. Conclusions: An important number of vessels (around 40%) that can be found in tumor area are neoformation vessels, this concept being an important assessment for the choice of the correct and effective treatment in colorectal adenocarcinoma.


Keywords: angiogenesis, colorectal cancer, CD31, CD34, CD105



Corresponding: Ionela Cristina Deliu, PhD Student, Department of Gastroenterology, University of Medicine and Pharmacy of Craiova, 2, Petru Rares St., Craiova, Romania; e-mail: cristina_umf@yahoo.com


DOI 10.12865/CHSJ.41.02.09 - Download PDF
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