CHSJ

Curr Health Sci J, vol. 37, no. 2, 2011

Immunohistochemical Aspects of Endometrium Hyperplasias in Perimenopause

[Original paper]

DANIELA ILIE(1),CLAUDIA VALENTINA GEORGESCU(2), CRISTIANA SIMIONESCU(3), ANCA DANIELA BRAILA(4), M. BRAILA(4)


(1) Department of Obstetrics and-Gynecology, Emergency County Hospital Slatina,
(2) Department of Pathology, Emergency University Hospital, Craiova,
(3) Department of Pathology, University of Medicine and Pharmacy, Craiova;
(4) Department of Obstetrics and-Gynecology, University of Medicine and Pharmacy, Craiova


Abstract:

The present study presents a comparative study of 30 diagnosed cases of endometrial hyperplasia at patients with menopause. The study group was compared with a group with well-differentiated endometrial carcinoma (G1) and a group with normal endometrium. The antibodies studied were represented by markers for hormone receptors (estrogen receptors-ER and progesterone receptor-PR), proliferation marker (Ki-67), epithelial membrane antigen (EMA) and p53 oncoprotein. ER and PGR values in hyperplasia have intermediate values, between the values of these receptors in normal proliferative endometrium and the ones of typical G1 endometrial carcinoma. Mitotic activity decreased with the increasing degree of hyperplasia, likely because the estrogen receptors have been repressed by a number of cofactors. The EMA immune-marking pattern seems to be useful in differentiating hyperplastic aspects without atypical markings. However, EMA cannot distinguish between complex atypical hyperplasia and endometrial carcinoma (G1). The correlation of results, obtained at p53 marking with the ones obtained for cell proliferation, suggests that the presence of wild type p53 protein accounts for the decrease in cellular proliferative activity with the increase of endometrial hyperplasia. Immunohistochemistry accounts for the same response to hormonal therapy in cases of endometrial hyperplasia but does not allow selection of cases presenting atypical hyperplasia, which are likely to develop subsequently into endometrial carcinoma.


Keywords: endometrial hyperplasia, menopause, immunohistochemistry



Corresponding: Daniela Ilie MD, PhD student, Department of Obstetrics and-Gynecology, Emergency County Hospital Slatina, email:dr_danailie@yahoo.com


DOI 10.12865/CHSJ.37.02.06 - Download PDF
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