CHSJ

Curr Health Sci J, vol. 39, no. 4, 2013

Chromatographic Analysis of the Flavonoids from Robinia Pseudoacacia Species

[Original Paper]

D. CLINA(1), N. KINGA OLAH(2), E. PTRU(3), A. DOCEA(1), H. POPESCU(4), M.V. BUBULICA(1)


(1)University of Medicine and Pharmacy of Craiova, Faculty of Pharmacy;
(2)Department of Research & Development, PlantExtrakt, Laboratories of Phytotherapy and Homeopathy;
(3)University of Medicine and Pharmacy of Craiova, Faculty of Medicine;
(4)University of Medicine and Pharmacy “Iuliu Hatieganu”, Cluj, Faculty of Pharmacy


Abstract:

The purpose of this study was the qualitative and quantitative analysis of flavonoids from Robinia pseudoacacia using two different techniques of analysis: Thin Layer Chromatography (TLC) and TLC coupled with photo-densitometry. The results obtained by chromatographic analysis showed a higher concentration of flavonoids in flowers than in leaves. The flowers harvested in the plains have a higher concentration of hyperoside (0.9 mg/mL) compared with the flowers collected from the hills (0.54 mg/mL). The leaves are richer in ruthoside (0.98 mg/mL) compared with the flowers


Keywords: TLC, TLC-photodensimetry, flavonoids, Robinia pseudoacacia



Corresponding: Daniela Calina, PhD, University Lecturer, 2-4 Petru Rares Street, 200638 Craiova, Romania, e-mail calinadaniela@gmail.com, tel/fax: 0040251533713


DOI 10.12865/CHSJ.39.04.07 - Download PDF
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