CHSJ

Curr Health Sci J, vol. 42, no. 2, 2016

Incidence and Antifungal Susceptibility of Candida Albicans Infections

[Original Paper]

ANCA UNGUREANU(1), ALICE ELENA GAMAN(1), ADRIANA TURCULEANU(1), MIHAELA MITROI(2), A.I. DROCAS(3), MARIA DOBRITOIU(4), D.O. ALEXANDRU(5), CORINA VASILE(6)


(1)Department of Bacteriology-Virusology- Parasitology, Faculty of Medicine, University of Medicine and Pharmacy of Craiova,
(2)Department of Otorhinolaryngology. Faculty of Dental Medicine, University of Medicine and Pharmacy of Craiova,
(3)Department of Urology, Faculty of Medicine, University of Medicine and Pharmacy of Craiova,
(4)Department of Oncology, Emergency County Hospital Craiova,
(5)Department of Medical Informatics and Biostatistics, University of Medicine and Pharmacy of Craiova,
(6)Student, Faculty of Medicine, University of Medicine and Pharmacy of Craiova


Abstract:

INTRODUCTION. Candida albicans is the most common inhabitant of the skin, mouth, vagina and gastro intestinal tract of human beings. One of the major reasons for the increase in Candida infection is the development of its resistant strains due to drugs used in the treatment of candidiasis. MATERIALS AND METHODS We studied 4027 samples collected from patients in various wards of the Emergency County Hospital Craiova, Romania between 2014-2015. The specimens were: pharyngeal exsudates, sputum, tracheal secretions, skin secretions, stools, ear secretions, urine, vaginal secretions. All the specimens were transported to the microbiology laboratory and cultured within 3 to 4 h of collection. Among the 4027 samples, 652 showed culture characteristics similar to Candida albicans.The samples were inoculated under sterile conditions using Sabouraud culture media, a medium designed to inhibit bacterial growth and allow the development of fungi. Antifungal Susceptibility Testing was performed by disc diffusion according to CLSI 2014 guidelines using: clotrimazole, ketoconazole, miconazole, econazole, amphotericine B, fluorocytozine, nistatin. RESULTS AND DISCUSSION In our study group the urocultures and dermatological products have a high infection rate, between 100% to 70%, in contrast, we find evidence of secretion ear (3.13%) and the throat swab (9.33%). Various resistant levels were detected against antifungal drugs but, complete resistance to 5 – Fluorocitozina (100%), and the organisms showed highly sensitive to Cotrimazol si Ketoconazol (100%). In the case of Miconazol 256 (39, 26%), Econazol, 215 (32,98%), Amphotericinei B, 230(35,28%). Nystatin 329 (50,46%). CONCLUSIONSElucidating these mechanisms may provide new foundations for antifungal chemotherapy and can present an exciting challenge for the future investigations. Candida albicans infections are present and diverse clinical pathology.


Keywords: incidence, antifungal susceptibility, Candida albicans, Cotrimazol, Ketoconazol



Corresponding: Anca Ungureanu, University of Medicine and Pharmacy of Craiova, Petru Rares St, No.2, Craiova 200349, Romania; e-mail: ancaungureanu65@yahoo.com


DOI 10.12865/CHSJ.42.02.08 - Download PDF
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