CHSJ

Curr Health Sci J, vol. 43, no. 1, 2017

In vitro Research of the Concentration Dependence of Effect of Adrenaline on Platelets Aggregation

[Original Paper]

E. MATI(1), ALICE PIPEREA-SIANU(1), I. SARBU(1), A. CROITORU(1), STILIYANA BORISOVA(1)


(1)“Carol Davila” University of Medicine and Pharmacy, Faculty of Pharmacy, Doctoral School, Bucharest, Romania


Abstract:

Purpose. Paper intended to present experimental evidences that adrenaline has a direct effect of inducing platelets aggregation in the concentration range 1-8µM. Material/ Methods. Platelet rich plasma from patients of Colentina Clinical Hospital, following an informed consent. The platelet rich plasma (PRP) was prepared by centrifuging the anticoagulated sample at 200 G for 10 minutes. Aggregation was evaluated by optical aggregometry, classical method of Born, using Helena PACKS-4 Aggregometer. Results. The curves transmission light-time followed the structure: a lag-time, a first phase aggregation, more or less linear, defined by a “Slope 1”, a second wave of aggregation defined by “slope 2” and a “saturation” phase. Slope 1 increases with the concentration of adrenaline. The second slopes of the aggregation curves, maximum aggregation and areas under curves depended linear on adrenaline concentration. Conclusions. Adrenaline, in concentrations in the 1-8µM, induce aggregation of human platelets from platelet rich plasma. Linear regression models for slope and area were practically identical suggesting a rather unique than biphasic mechanism of action of adrenaline during the time course of aggregation.


Keywords: Adrenaline, Platelet Aggregation, DLVO



Corresponding: Alice Piperea-Sianu, "Carol Davila" University of Medicine and Pharmacy, Faculty of Pharmacy, Doctoral School, 6 Traian Vuia Str., 020956, Bucharest, Romania; e-mail: alice_piperea@yahoo.com


DOI 10.12865/CHSJ.43.01.06 - Download PDF
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