CHSJ

Curr Health Sci J, vol. 43, no. 1, 2017

Immunohistochemical Study of Sentinel Lymph Node in Colon Cancer

[Original Paper]

MARIA CRISTINA SFECLAN(1), C.S. MIREA(1), M.C. CIORBAGIU(1), ALINA MARIA VILCEA(2), .V. OBLEAGA(1), V. PADUREANU(3), D. CARTU(4), T.S. TENEA-COJAN(5), E. MORARU(1), I.D. VILCEA(1)


(1)The 2nd Department of Surgery, Faculty of Medicine, University of Medicine and Pharmacy of Craiova, Romania,
(2)Department of Dermatology-Venerology, Faculty of Medicine, University of Medicine and Pharmacy of Craiova, Romania,
(3)The 2nd Department of Internal Medicine, Faculty of Medicine, University of Medicine and Pharmacy of Craiova, Romania,
(4)The 1st Department of Surgery, Faculty of Medicine, University of Medicine and Pharmacy of Craiova, Romania,
(5)The 4th Department of Surgery, Faculty of Medicine, University of Medicine and Pharmacy of Craiova, Romania


Abstract:

Identification of sentinel lymph node (SLN) in colon cancer is very important in order to increase the accuracy of lymph node staging. The number of examined lymph nodes represents a significant predictor of survival. This study aims to show the importance of SLN histological and immunohistochemical examination in adjuvant oncological treatment. The study includes 23 patients with colon cancer (44% women and 56% men) who came in our clinic for surgical intervention. In all cases, the SLN was identified and prepared for histological examination. In 13 of the cases, micrometastases were found on haematoxylin-eosin (HE) staining, there were 5 cases with positive immunohistochemistry using antibodies anti-p53, anti-VEGF-C, anti-CD34, and 5 cases with SLN negative both for HE and immunohistochemistry. Altogether we had a detection rate of 92%, an accuracy of 78,2%, a sensitivity of 90%, a false negative rate of 10% and a negative predictive value of 71,4%, good values according to the literature. Four (17,3%) patients had micrometastases exclusively in the sentinel lymph node, after performing additional histological examination, using multilevel section and immunohistochemistry. After assessing the SNL on our patients, we concluded that it is a reproducible practice for lymph node analysis.


Keywords: Immunohistochemistry, sentinel lymph node, colon cancer



Corresponding: C.S. Mirea, The 2nd Department of Surgery, Faculty of Medicine, University of Medicine and Pharmacy of Craiova, Romania, e-mail: mirea_cecil@yahoo.com


DOI 10.12865/CHSJ.43.01.07 - Download PDF
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