CHSJ

Curr Health Sci J, vol. 43, no. 3, 2017

Interleukin-8 mRNA Expression in Locally Advanced Colorectal Cancer Patients

[Original Paper]

A. BARBALAN(1), I. STREATA(2), E.T. IVAN(3), I. CHERCIU(4), V. SURLIN(5), M. IOANA(2), A. SAFTOIU(6)


(1)PhD Student, University of Medicine and Pharmacy of Craiova, Romania;
(2)Human Genomics Laboratory, University of Medicine and Pharmacy of Craiova, Romania;
(3)Research Center of Gastroenterology and Hepatology Craiova, University of Medicine and Pharmacy of Craiova, Romania;
(4)Department of Internal Medicine, County Clinical Emergency Hospital of Craiova;
(5)First Clinic of Surgery, County Clinical Emergency Hospital of Craiova;
(6)Department of Endoscopy, Gastrointestinal Unit, Copenhagen University Herlev Hospital; Research Center of Gastroenterology and Hepatology Craiova, University of Medicine and Pharmacy of Craiova, Romania


Abstract:

Purpose: Interleukin-8 (IL-8) has been proven to promote progression of malignant tumours and control angiogenesis processes. We aim to determine and compare interleukin-8 (IL-8) gene expression level in colorectal tumors (CCR) and peritumoral samples obtained through endoscopic biopsy. Material and methods: Total mRNA was obtained from both tumoral and peritumoral tissue samples collected from patients diagnosed with colorectal cancer. Through Quantitative Real Time PCR, IL-8 gene expression was assessed in both pathologic tissue and adjacent normal mucosa. Results: In our cohort, IL8 expression was higher in adjacent normal mucosa than in tumoral tissue, in all the samples. Further studies on larger groups are required to validate our results.


Keywords: Interleukin-8 mRNA, advanced colorectal cancer, endoscopy, preoperative analysis



Corresponding: A. Saftoiu, University of Medicine and Pharmacy of Craiova, Petru Rares St. No 4, 200456, Craiova, Romania, e-mail: adriansaftoiu@aim.com


DOI 10.12865/CHSJ.43.03.04 - Download PDF
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