CHSJ

Curr Health Sci J, vol. 43, no. 4, 2017

Isolation of Collagen from Marine Resources from the Black Sea

[Original Paper]

M. CHERIM(1), R. SIRBU(2), I. BELU(3)


(1)PhD-Student Pharmacyst, Doctoral School ”Carol Davila” University of Medicine and Pharmacy, Faculty of Pharmacy, Bucharest, Romania; 
(2)Faculty of Pharmacy, ”Ovidius” University of Constanta, Romania; 
(3)Faculty of Pharmacy, University of Medicine and Pharmacy of Craiova, Romania


Abstract:

Purpose: Evaluation of possibilities of obtaining type I fibrillar collagen from fish skin using the Black Sea marine resources and suitable cross-linking agent for this one to stay stable at body or room temperature. Materials and methods: Collagen has been extracted from the skin of the Grey mullet and Sturgeon by the acidic method with Acetic acid 0.5M. Cross-linking has been performed by using Tanic acid at 4-6oC. The rheological behaviour was determined by using a Haake VT 550 rheo-viscometer. Results: The collagen was isolated from the skin of Gray mullet and Sturgeon. (Acipenser gueldenstaedtii). Hydrogels have a pseudoplastic behavior. Tanic acid was used for cross-linking like a better alternative, eliminating the toxicity of glutaraldehyde. Conclusion. The yield of collagen extraction was higher for the Gray mullet skin, than Sturgeon. Pseudo-plastic behavior allows them to be successfully applied in the treatment of wounds. The isolated type I collagen may serve as an attractive alternative to mammalian collagen for biomedical and pharmaceutical applications.


Keywords: marine collagen, collagen hydrolysates, tissue regeneration



Corresponding: Ionela Belu, Faculty of Pharmacy, University of Medicine and Pharmacy of Craiova, Romania, e-mail: ionelabelu@gmail.com


DOI 10.12865/CHSJ.43.04.02 - Download PDF
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