CHSJ

Curr Health Sci J, vol. 45, no. 1, 2019

A Novel Method of Obtaining Platelet Rich Fibrin from Rats and Quantifying Platelet Count

[Original paper]

A.F. GRECU(1), D.C. GRECU(2), O. NICA(1), E.M. CIUCA(3), A. CAMEN(3), M.E. CIUREA(4)


(1)PhD Researcher, University of Medicine and Pharmacy of Craiova, Romania,
(2)Department of Orthopaedic and Traumatology Surgery, University of Pharmacy and Medicine of Craiova, Romania,
(3)Department of Oro-Maxilo-Facial Surgery, University of Medicine and Pharmacy of Craiova, Romania,
(4)Department of Plastic Surgery, University of Medicine and Pharmacy of Craiova, Romania


Abstract:

Background and objectives: Rat experimentation is the first line of research by which a medical hypothesis is usually tested. Platelet Rich Fibrin (PRF) is a relatively new bio-material that has shown promise to enhance healing in the field of bone research and tissue engineering. In order to perform PRF based experiments on rats, a proper protocol of obtaining PRF from rats needs to be established. Materials and Methods: 35 Wistar rats were used to obtain PRF by using cardiac puncture blood draw and quick subsequent centrifugation. The PRF samples were analyzed and compared to standard literature PRF composition. Results: PRF samples analysis showed persistent results pertaining to known PRF composition. Conclusions: Our experiment has shown that our protocol of obtaining PRF is capable of providing high quality PRF from rats.


Keywords: Platelet-rich fibrin, tissue engineering, proof of concept study, experimental animal models, platelet count



Corresponding: Oliviu Nica, University of Medicine and Pharmacy of Craiova, str. Petru Rares, no.2, 200349, e-mail: oliviu.nica@gmail.com


DOI 10.12865/CHSJ.45.01.14 - Download PDF
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