Curr Health Sci J, vol. 47, no. 2, 2021

Heterogeneity in the Number of Astrocytes in the Central Nervous System after Peritonitis

[Original Paper]

Alexandra Daniela Rotaru-Zavaleanu(1,2), Alexandru Ionu Neacu(2,3), Alexandru Cojocaru(2,4), Eugen Osiac(2,3), Dan Ionut Gheonea(1)


(1)Department of Gastroenterology, University of Medicine and Pharmacy of Craiova, Romania,
(2)Experimental Research Center for Normal and Pathological Aging, University of Medicine and Pharmacy of Craiova, Romania,
(3)Department of Biophysics, University of Medicine and Pharmacy of Craiova, Romania,
(4)Department of Physiology, University of Medicine and Pharmacy of Craiova, Romania


Abstract:

Sepsis remains a major medical emergency that describes the body's systemic immune response to an infectious process and can lead to end-stage organ dysfunction and death. Clinical studies have introduced the concept of sepsis associated encephalopathy, which seems to have a plethora of cellular and molecular triggers starting from systemic inflammatory cytokines, blood-brain barrier (BBB) rupture, microscopic brain injury, altered cerebral circulation, neurotransmission, or even metabolic dysfunction. The purpose of our study is to reproduce the sepsis model previously described using the cecal ligature and puncture (CLP), and to take a closer look to the acute modifications that occur on cellular level when it comes to the brain-blood-barrier of the mice with systemic inflammation. After a rapid systemic response to peritonitis, we show a heterogeneity in astrocytic response within different cortical structures; hippocampus having the longest change in the number of GFAP+cells, while no difference was seen in the number of cortical astrocytes. With even more increasing roles of astrocytes in different pathologies, the relation between sepsis and astrocytes could prove a valuable in discovering new therapy in sepsis


Keywords:
Sepsis, atrocytic response, peritonitis



Corresponding:
Alexandru Cojocaru, Experimental Research Center for Normal and Pathological Aging, Department of Physiology, University of Medicine and Pharmacy of Craiova, Romania, e-mail: cojo.alexandru92@gmail.com


DOI 10.12865/CHSJ.47.02.03 - Download PDF